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Ronaldo had shot Juventus in the European quarterfinals with a win back on Atletico Madrid last Tuesday.
Without him the team collapsed to a 2-0 defeat but remains comfortably in the race for an eighth consecutive league title.
The advance of Juventus has been reduced to 15 points per Napoli who won by winning 4-2 against Udinese after goalkeeper David Ospina s' collapsed on the ground after suffering a head injury.
The ready Arsenal Guardian was then given all clear after a scanner to a Naples hospital.
Juventus had won 24 of their 27 previous matches in Serie A this season with three draws, including a 1-1 in the deadlock Genoa in Turin.
"It's not a tragedy, but I am sorry because we wanted to stay undefeated ", said Allegri.
"You can not play all the games to your full potential. Champions League match certainly weighed heavy ".
It was the first defeat of the league for Side of Allegri since April 2018 against Napoliand first start since losing to Sampdoria in November 2017.
– It's time to unplug & # 39; –
The champions of Italy had already been beaten this season by Atalanta in the Coppa Italia and lost in the Champions League at Young boys in group stage and Atletico Madrid in the last match 16.
"The defeat could be good for us, because maybe we thought we already had the league in our pockets and we still need to get five wins," continued Allegri.
"A bit like the Coppa Italia defeat at Atalantawe have chosen the right game to lose, because we are just before the break from international duty, so we have time to unplug and rest. "
Former Juventus midfielder Stefano Sturaro, 26, plays his first match after ten months of absence with a tendon injury. He did not score until two minutes after getting off the bench. Another substitute Goran Pandev sealed victory for Genoa with ten minutes to go.
"I am happy for him (Sturaro) because he has suffered a lot and nowadays he changed the game, "I said Allegri.
Napoli, meanwhile, rallied after leaving a two-goal lead just before The scary collapse of Ospina.
The Colombian international collided with Udinese striker Ignacio Pussetto minutes in the game, before collapsing just before half-time.
"Fortunately, nothing serious, he is aware, they did a negative scanner, he will remain under surveillance tonight," said the coach. Carlo Ancelotti.
Amin Younes wrapped up in his first goal in Serie A after 17 minutes of play, with Jose Callejon connect with a Dries Mertens cross for the second on 26 minutes.
But Kevin Lasagna and Seko Fofana at two o'clock Ospina Six minutes after the half-hour mark, the Colombian looked disgusted after his fourth-minute collision.
Alex Meret replaced Ospina with Napoli pulling towards the front when Arkadiusz Milik headed in a Callejon 57 minutes, with Mertens seal the three points on 69 minutes.
– Derby inter board –
In the midst of the drama and tension at the San Siro, Inter Milan outdated AC Milan to consolidate their hopes of a Champions League back next season, ending Undefeated by Gennaro Gattuso going back to December 22nd.
AC Milan did not play in the Champions League since 2014.
Matias Vecino found a way after just three minutes to the San Siro, a few days after his team Europa League exit.
Stefan from Vrij added the second on 51 minutes and Lautaro Martinez placed in the third from the penalty spot.
For kite, Tiemoue Bakayoko directed after 57 minutes with Mateo Musacchio react quickly to finish a rebound (71).
Enter had won the reverse encounter thanks to his ex-captain The last winner of Mauro Icardi but Argentina has not played for more than a month in a contractual dispute.
Luciano Spalletti's side are now two points ahead from Milan.
Roma, are fifth, four points behind the Champions League places after a 2-1 defeat to SPALwith Lazio two points in sixth after defeating parma 4-1.
Atalanta are equal on the points with Lazio after being held 1-1 by the bottom club Chievo.
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