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Jie Dai,1 * Kang Lin,2, * Yan Huang,3 Yan Lu,4 Wen-Qi Chen,1 Xiao-Rong Zhang,1 Bang-Shun He,5 Yu-Qin Pan,5 Shu-Kui Wang,2 Fan of Wei-Xin4
1Department of Dermatology, Nanjing First Hospital, Nanjing Medical University, Nanjing, China; 2Department of Laboratory Medicine, Nanjing First Hospital, Nanjing Medical University, Nanjing, China; 3Department of Ultrasound, Nanjing First Hospital, Nanjing Medical University, Nanjing, China; 4Department of Dermatology, the first affiliated hospital of the Nanjing Medical University, Nanjing, China; 5General Clinical Research Center, Nanjing First Hospital, Nanjing Medical University, Nanjing, China
*These authors participated in this work in equal parts
Context: Basal cell carcinoma (BCC) is a common malignant tumor of skin cancer with high morbidity. The objective of this study was to identify genes and critical pathways related to CBC carcinogenesis and to better understand the molecular mechanisms underlying CBC.
Materials and methods: The gene expression profiles of GSE7553 and GSE103439 were downloaded from the Gene Expression Omnibus database with 19 tumors and 6 normal skin tissues. Differentially expressed genes (DEGs) were screened between CCB samples and normal tissues, followed by a gene ontology and enrichment analysis of the Encyclopedia pathway. genes and genomes of Kyoto. Subsequently, a network of protein-protein interactions (PPI) was constructed for these DEGs and a module analysis was performed.
Results: A total of 313 DEG was obtained. Of these, 222 genes were upregulated and 91 genes were downregulated. An enrichment analysis indicated that the positively regulated genes were significantly enriched in the cell cycle and in mitosis, while the negatively regulated genes were mainly associated with the metabolic process of unsaturated fatty acids and cell differentiation. In addition, TOP2A, CDK1, and CCNB1 have been identified as the top three pivotal genes graded in the PPI network. In parallel, three subnetworks were derived, indicating that these DEGs were significantly enriched in pathways, including "cell cycle", "extracellular matrix-receptor interaction", "basal cell carcinoma" and "Hedgehog signaling pathway".
conclusions: The new DEGs and critical pathways identified in this study could play a central role in the carcinogenesis of CBC and indicate more molecular targets for the treatment of CBC.
Keywords: basal cell carcinoma, differential expression genes, enrichment analysis, bioinformatic analysis
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