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Dressings based on nanofibers loaded with vitamin D stimulate the production of an antimicrobial peptide, a key step in the fight against surgical site infections, or SSI.
Findings of Oregon State University researchers and other collaborators Wednesday Nanomedicine is important because ISOs are the most common health care-associated infection and cause widespread human suffering and economic loss.
Every year in the United States, nearly 300,000 patients 30 days of their operation – representing about $ 10 billion in additional health costs – and more than 13,000 of these people die.
The researchers used electrospinning to prepare dressings containing the bioactive form of vitamin D: 1,25-dihydroxyvitamin D3, or 1,25 (OH) 2D3.
"Electrospinning is a versatile, simple, cost-effective and reproducible technique for generating long fibers with nanometer-sized meters," said Adrian Gombart, co-author and professor of biochemistry and biophysics at the College. OSU Science. "Electrofilar nanofiber dressings offer significant advantages over hydrogels or sponges for local drug delivery, providing several functional and structural benefits, including scar-free healing."
The dressings created by the researchers proved to be able to provide vitamin D for four weeks, they significantly induced the production of a peptide, hCAP18 / LL37, which kills microbes by disrupting their membranes. [19659002] "In previous research on nanofiber-based sutures, we used the inactive form of vitamin D D3 – hydroxyvitamin and a toll receptor ligand that activated the cells to convert 25D3 to the bioactive form, 1,25D3 ", said the other co-corresponding author, Jingwei Xie, an assistant professor at the same time. University of Nebraska Medical Center. "Here we bypassed that and went straight to the active form. The dressing just released him and he started activating the vitamin D target genes, one of which produced the LL37 peptide."
Because dressings improve innate immune responses rather than containing conventional single target antimicrobial compounds, they are less likely to contribute to drug resistance. The dressings were tested on human skin (taken from plastic surgery patients) in a culture dish, as well as in vitro with keratinocyte and monocyte cell lines, and in vivo in a murine model
. "said co-author Arup Indra, associate professor of pharmacy at OSU." It seems we can induce genes into a model system and now we can begin to consider healing and infection. "
In addition to Indra, Xie and Gombart, senior researcher at the Linus Pauling Institute of OSU, the collaboration included Gitali Indra, associate professor of pharmacy research OSU, and scientists from the USSR. University of California San Diego and VA Nebraska-Western Health System Iowa
"Our study suggests that 1,25D3-induced hCAP18 expression by these nanofiber dressings is a step toward improving healing. sores, "said Gitali Indra.
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