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New York, Jul 1 (IANS): A new method of molecular imaging can monitor the success of gene therapy in all areas of the brain that can allow doctors to effectively attack it to brain diseases such as Parkinson's disease and Alzheimer's disease. A recent study suggests that reporter gene systems have been a key tool in molecular imaging for a number of years, but they have not allowed monitoring of all areas of the brain.
A new positron The Transmitted Tomography Probe / Probe System (PET) allows for the first time noninvasive monitoring of the level and location of gene expression in all regions of the world. brain
"It is difficult to find a reporter gene. and an imaging agent that can be used in all areas of the brain with a high signal-to-background ratio, "said Thomas Haywood of Stanford University, California.
" 18F -DASA-23 is a new radiotracer, or reporter probe, that is capable of creating the blood-brain barrier and targeting the pyruvate kinase M2 protein in the central nervous system with minimal endogenous expression in the brain. "
" This allows us to monitor the expression of reporter genes and ultimately the expression of therapeutic genes in all regions. The researchers mentioned that the radiotracer has recently undergone in vitro badays in the United States. for the early detection of the therapeutic response in glioblastoma, the most aggressive cancer that begins in the brain
. study, presented at the annual meeting SNMMI 2018, after validation of the utility of pyruvate kinase M2 (PKM2) as a reporter gene PET, mice were infected with a virus containing the gene and then subjected to imaging of the 18F-DASA-23 radiotracer over a two-month period to observe the increase in PKM2 expression over time.
The results, confirmed by studies of 18F-DASA-23 uptake and mRNA badysis, showed a good correlation between PKM2 and radio. trace.
Other badyzes showed an increase in PKM2 expression in infected mice compared to controls.
These encouraging data suggest PKM2 has the potential to be developed into a PET reporter gene system for imaging gene therapy in the field. The researchers said the central nervous system was reached
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